Children who were negative in all three laboratory tests were not investigated further, as they Nike Blazer White UK
had a very low probability of having coeliac disease, and biopsy was considered unethical in such children.The samples were number coded and sent to Heim Pl Children's Hospital, Budapest, Hungary. To check for correct sampling and to perform an Nike Blazer Mid Suede Mens
interobserver comparison between the nurses and the laboratory, the nurses also submitted any diluted blood left over from the rapid test.
Parents gave written informed consent to the blood sampling and screening. We sought separate consent for the confirmatory Nike Blazer Print UK Mens
biopsy in children with positive test results.Rapid coeliac antibody testing Nurses used the Biocard coeliac disease test kit (AniBiotech) and evaluated results after five minutes but not later than 10 minutes, as instructed by the manufacturer. This immunochromatographic test detects IgA antibodies to tissue transglutaminase in whole blood with the aid of transglutaminase antigen contained within the erythrocytes in the sample being tested.141516 In brief, the blood sample is taken into a 10 l capillary, which is then inserted into a tube with haemolysing buffer to liberate the transglutaminase from the erythrocytes, and three drops are added to the test cassette.
20 We also asked the parents of patients with coeliac disease to answer the questionnaire after Nike Blazer Red UK
the children had spent six months on a gluten free diet.Statistical analysisWe calculated the sensitivity, specificity, positive predictive value, and negative predictive value of the White Nike Blazer Low
rapid tests and compared them with those obtained for laboratory antibody tests and biopsy; 95% confidence intervals were determined by the Wilson method. We compared median antibody titres before and after dietary treatment by means of the Wilcoxon signed ranks test.
Haemoglobin values and body mass index before and after the gluten free diet were compared by means of the paired t test.ResultsScreening resultsIn White Nike Blazer Print Womens
total, 3518 births were registered in the county between 1 June 1998 and 31 May 1999. Of these children, 2690 (76.5%) joined the study. Main reasons for non participation were refusal of the parents (7%) or nurses (8%) and clashes between the nurses' working schedule and the children's summer holidays (8.5%).
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